Creatine supplementation in trained rats causes changes in myenteric neurons and intestinal wall morphometry

Creatine is widely used by athletes as an ergogenic resource. The aim of this study was to evaluate the influence of creatine supplementation on the duodenum of rats submitted to physical training. The number and myenteric neuronal cell bodies as well mucosal and muscular tunic morphometry were evaluated. Control animals received a standard chow for 8 weeks, and the treated ones received the standard chow for 4 weeks and were later fed with the same chow but added with 2% creatine. Animals were divided in groups: sedentary, sedentary supplemented with creatine, trained and trained supplemented with creatine. The training consisted in treadmill running for 8 weeks. Duodenal samples were either processed for whole mount preparations or for paraffin embedding and hematoxylin-eosin staining for histological and morpho metric studies of the mucosa, the muscular tunic and myenteric neurons. It was observed that neither creatine nor physical training alone promoted alterations in muscular tunic thickness, villus height or crypts depth, however, a reduction in these parameters was observed when both were associated. The number of myenteric neurons was unchanged, but the neuronal cell body area was reduced in trained animals but not when training and creatine was associated, suggesting a neuroprotector role of this substance.

O exercicio de final de semana contribui para o controle das dislipidemias em ratos adultos machos alimentados com dieta rica em gorduras / The weekend exercise contribute to the control in the hiperlipidaemias in adults male rates feeding with lipids diet

Sabe-se que o sedentarismo, associado a uma dieta nao balanceada tem grande importancia para o desenvolvimento de doencas cronico degenerativas. Por outro lado, o exercicio fisico tem sido um meio utilizado para o controle dessas doencas. Entretanto, pouco foi relatado sobre efeitos do exercicio de final de semana nocontrole das dislipidemias. Assim, o objetivo deste estudo foi observar respostas adaptativas ao exercicio de final de semana sobre o metabolismo das lipoproteinas em ratos machos alimentados com dieta hiperlipidica. Foram divididos em grupos 96 ratos machos adultos: dieta normocalorica (N) e hipercalorica (H), sedentario (S), treinado continuo (TC) e treinado de fim de semana (TFS), 5 e 2 sessoes semanais/90minutos/dia, respectivamente, por natacao. Durante todo o periodo experimental foram controlados: o peso e o consumo alimentar em gramas. Foram coletados, pesados e congelados o plasma (P), os tecidos adiposos barncos retropeitoneal (RET) e epididimal (EPI), tecido adiposo marron (TAM), musculo gastrocnemio (GAST), figado (FIG) e coracao (COR), para posteriores analises. Foram considerados significativos os valores de p<-0,05 a partir da analise de variancia `Anova Tree Way`. O treinamento de final de semana promoveu diminuicao significativa nos triglicerideos plasmaticos em animais hipercaloricos (SH 180,7+-13,9 vs TFSH97,2+-8,3). Os dois tipos de treinamento foram importantes no controle das dislipidemias em ratos alimentados com dieta hipercalorica, sendo observado aumento na concentracao de HDL colesterol em relacao aos respectivos animais sedentarios (SH 26,5+-2,0 vs TCH 35,7+-6,2 e TFSH 37,1+-1,2)

Aislamiento de Plesiomonas shigelloides en un paciente pediátrico / Isolation of Plesiomonas shigelloides from a pediatric patient

We report the isolation of P. shigelloides in the stools of a child with acute diarrhea living in the surroundings of Buenos Aires who usually drank untreated water. P. shigelloides has been implicated as an etiological agent in many sporadic cases of diarrhea, probably associated with drinking untreated water, eating uncooked seafood or foreign travel.